Fusion and biochemical expression of membrane receptors in foreign living cells.

Therapeutic Approaches
Other

Abstract

Successful transplantation of cell surface molecules from the membranes of one cell type to recipient cells of a different type is described. Plasma membranes purified from donor cells were fluoresceinated and fused to recipient cells using poly(ethylene glycol) and the fate of the transplanted membrane components was followed by fluorescence microscopy. In approximately 100 min the 'foreign' membrane components were seen to cluster and internalise. During this time, judged by the criteria of hormonal stimulation and immune cytotoxic killing, the cell surface of the recipient cell mimicked the cell surface phenotype of the donor cell.

Authors

Balakrishnan, Krishna; Lewis, Janine; Mehdi, S Q; McConnell, H M;

Keywords

  • Adenylyl Cyclases/ metabolism
  • Animals
  • Cell Fusion
  • Cell Membrane/ transplantation
  • HLA Antigens/ analysis
  • Histocompatibility Antigens/ analysis
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Microscopy, Fluorescence
  • Polyethylene Glycols
  • Receptors, Cell Surface/ analysis
  • Receptors, Thyrotropin
  • Surface Properties

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