High-Throughput and High-Content Micronucleus Assay in CHO-K1 Cells.

Methods Development

Abstract

Visualization of micronuclei induction by chemicals and drugs enables measurement of possible compound genotoxicity. A loss of entire chromosome or a fragment of chromosome can lead to formation of micronuclei (MNi). The in vitro micronucleus assay can be conducted using nuclear dyes with high-content imaging platforms. This chapter describes the cytochalasin block method of measuring micronuclei in CHO-K1 cell lines.

Authors

Shahane, Sampada A; Nishihara, Kana; Xia, Menghang;

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