A Novel Chemotherapeutic Agent to Treat Tumors with DNA Mismatch Repair Deficiencies.

Therapeutic Approaches

Abstract

Impairing the division of cancer cells with genotoxic small molecules has been a primary goal to develop chemotherapeutic agents. However, DNA mismatch repair (MMR)-deficient cancer cells are resistant to most conventional chemotherapeutic agents. Here we have identified baicalein as a small molecule that selectively kills MutSα-deficient cancer cells. Baicalein binds preferentially to mismatched DNA and induces a DNA damage response in a MMR-dependent manner. In MutSα-proficient cells, baicalein binds to MutSα to dissociate CHK2 from MutSα leading to S-phase arrest and cell survival. In contrast, continued replication in the presence of baicalein in MutSα-deficient cells results in a high number of DNA double-strand breaks and ultimately leads to apoptosis. Consistently, baicalein specifically shrinks MutSα-deficient xenograft tumors and inhibits the growth of AOM-DSS-induced colon tumors in colon-specific MSH2 knockout mice. Collectively, baicalein offers the potential of an improved treatment option for patients with tumors with a DNA MMR deficiency. Cancer Res; 76(14); 1-9. ©2016 AACR.

Authors

Zhang, Yongliang; Fox, Jennifer T; Park, Young-Un; Elliott, Gene; Rai Bantukallu, Ganesha; Cai, Mengli; Sakamuru, Srilatha; Huang, Ruili; Xia, Menghang; Lee, Kyeryoung; Jeon, Min Ho; Mathew, Bijoy P; Park, Hee Dong; Edelmann, Winfried; Park, Chan Young; Hong, Sung You; Maloney, David; Myung, Kyungjae;

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