Cre dependent DREADD (designer receptors exclusively activated by designer drugs) mice.

Therapeutic Approaches

Abstract

DREADDs, designer receptors exclusively activated by designer drugs, are engineered G protein-coupled receptors (GPCR) which can precisely control GPCR signaling pathways (for example, Gq, Gs and Gi). This chemogenetic technology for control of GPCR signaling has been successfully applied in a variety of in vivo studies, including in mice, to remotely control GPCR signaling, for example, in neurons, glia cells, pancreatic beta-cells, or cancer cells. In order to fully explore the in vivo applications of the DREADD technology we generated hM3Dq and hM4Di strains of mice which allow for Cre recombinase-mediated restricted expression of these pathway-selective DREADDs. With the many Cre driver lines now available, these DREADD lines will be applicable to studying a wide array of research and preclinical questions. This article is protected by copyright. All rights reserved.

Authors

Zhu, Hu; Aryal, Dipendra K; Olsen, Reid H J; Urban, Daniel; Swearingen, Amanda; Forbes, Stacy; Roth, Bryan L; Hochgeschwender, Ute;

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