KDM4/JMJD2 Histone Demethylase Inhibitors Block Prostate Tumor Growth by Suppressing the Expression of AR and BMYB-Regulated Genes.

Therapeutic Approaches

Abstract

Histone lysine demethylase KDM4/JMJD2s are overexpressed in many human tumors including prostate cancer (PCa). KDM4s are co-activators of androgen receptor (AR) and are thus potential therapeutic targets. Yet to date few KDM4 inhibitors that have anti-prostate tumor activity in vivo have been developed. Here, we report the anti-tumor growth effect and molecular mechanisms of three novel KDM4 inhibitors (A1, I9, and B3). These inhibitors repressed the transcription of both AR and BMYB-regulated genes. Compound B3 is highly selective for a variety of cancer cell lines including PC3 cells that lack AR. B3 inhibited the in vivo growth of tumors derived from PC3 cells and ex vivo human PCa explants. We identified a novel mechanism by which KDM4B activates the transcription of Polo-like kinase 1 (PLK1). B3 blocked the binding of KDM4B to the PLK1 promoter. Our studies suggest a potential mechanism-based therapeutic strategy for PCa and tumors with elevated KDM4B/PLK1 expression.

Authors

Duan, Lingling; Rai Bantukallu, Ganesha; Roggero, Carlos; Zhang, Qing-Jun; Wei, Qun; Ma, Shi Hong; Zhou, Yunyun; Santoyo, John; Martinez, Elisabeth D; Xiao, Guanghua; Raj, Ganesh V; Jadhav, Ajit; Simeonov, Anton; Maloney, David J; Rizo, Josep; Hsieh, Jer-Tsong; Liu, Zhi-Ping;

Keywords

  • Animals
  • Cell Cycle/ drug effects
  • Cell Cycle/ genetics
  • Cell Cycle Proteins/ genetics
  • Cell Cycle Proteins/ metabolism
  • Cell Growth Processes/ drug effects
  • Cell Line, Tumor
  • Enzyme Inhibitors/ pharmacology
  • Gene Knockdown Techniques
  • Humans
  • Jumonji Domain-Containing Histone Demethylases/ antagonists & inhibitors
  • Jumonji Domain-Containing Histone Demethylases/ genetics
  • Jumonji Domain-Containing Histone Demethylases/ metabolism
  • Male
  • Mice
  • Mice, SCID
  • Prostatic Neoplasms/ drug therapy
  • Prostatic Neoplasms/ genetics
  • Prostatic Neoplasms/ metabolism
  • Prostatic Neoplasms/ pathology
  • Protein-Serine-Threonine Kinases/ genetics
  • Protein-Serine-Threonine Kinases/ metabolism
  • Proto-Oncogene Proteins/ genetics
  • Proto-Oncogene Proteins/ metabolism
  • Random Allocation
  • Receptors, Androgen/ biosynthesis
  • Receptors, Androgen/ metabolism
  • Xenograft Model Antitumor Assays

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